Cell Biology Laboratory
Av. Universidad #1115
Col. Linda Vista
47810, Ocotlán, Jalisco
México
| Address | Centro Universitario de la Ciénega (CUCI) Cell Biology Laboratory Av. Universidad #1115 Col. Linda Vista 47810, Ocotlán, Jalisco México |
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| Telephone | +52 (392) 9259400 - 48354 | |
| knauth (at) gmx . de |
| 10/89 – 03/96 | Studies of Biology and Chemistry at the Carl-von-Ossietzky University of Oldenburg, Germany |
| 24.9.1992 | Vordiplom (Bachelor) in Biology |
| 23.6.1993 | Vordiplom (Bachelor) in Chemistry |
| 28.3.1996 | Diplom (Master) in Biology |
| 09/96 – 08/99 | PhD student at the National Research Centre of Biotechnology (GBF) in Braunschweig, Germany |
| 3.3.2000 | Doctor rerum naturalium, Dr. rer. nat.: "Magna cum laude" |
| 08/94 | Practice (separating pigments from Cyanobacteria by HPLC) at the National Research Centre (GKSS) in Geesthacht, Germany |
| 03/95 – 03/96 | Diplom Thesis (Master): Water chemical and biological observations of the 1993 re-arisen shallow lake "Nonnensee" (Rügen) during the vegetation period 1995 |
| 04/96 – 08/96 |
Scientific Assistant in the environmetal project (GOAP) in the Ernst-Moritz-Arndt University in Greifswald, Germany, under the direction of Prof. Dr. Conrad |
| 09/96 – 08/99 | Scientific Assistant in the National Research Centre of Biotechnology (GBF) in Braunschweig, Germany, under the direction of Prof. Dr. Reichenbach. Realising the PhD thesis: "Studies on the mode of action of Ambruticin VS-3" (in German)  |
| 01/00 – 12/04 | Post doctorate at the Otto-von-Guericke University of Magdeburg, Germany, at the Institute for Biochemistry under the direction of Prof. Dr. Bohnensack Research topic: Protein trafficking mediated by the sorting nexin SNX17 |
| 01/05 – 01/06 | Researcher Titular "A" at the Research Centre CIATEJ in Guadalajara, Mexico. Function: applying for research projects the pharmaceutical area |
| since 02/06 | Research Professor Titular "B" at the University Centre of the Ciénega (CUCI), part of the University of Guadalajara. Function: - Giving lectures in Biochemistry, Astrobiology (Bachelor Pharmacy) and Cell Biology (Master) - Responsible of the Cell Biology Laboratory |
González A., N. Casillas, Z. López, O. Cervantes, P. Knauth, R. Hernández, A. Topete, S. Rosales, L. Quintero, J. Avila, X. Flores and M.E. Cano (2022): Tetrahydroxyquinone: a suitable coating for ferrofluids used in magnetic hyperthermia. Coatings, 12 ID081130: 14 pp.
López Z., M. Salazar, A. Femenia, G. Acevedo, J. Godínez, M.E. Cano and P. Knauth (2022): Dry but not humid thermal processing of Aloe vera gel promotes cytotoxicity on human intestinal cells HT-29. Foods, 11 ID050745: 14 pp.
Cervantes O., Z. Lopez, N. Casillas, P. Knauth, N. Checa, F. Cholico, R. Hernandez, L. Quintero, J. Avila and M.E. Cano (2022): A ferrofluid with surface modified nanoparticles for magnetic hyperthermia and high ROS production. Molecules, 27 ID020544: 21 pp.
Tinajero-Díaz E., D. Salado, C. Gonzalez, M. Martínez, Z. Lopez, J. Bravo, P. Knauth, F.Y. Flores, S.E. Herrera, R.E. Navarro, A. Cabrera, E. Krötzsch, Z.Y. García and R. Hernández (2021): Green metallic nanoparticles for cancer therapy: evaluation models and cancer applications. Pharmaceutics, 13 ID101719: 52 pp.
Knauth P., T. Espino and Z. López (2020): The Genus Phaseolus as a Source for ACE Inhibitory Peptides. In A. Cardador and C. Jiménez (eds.), Angiotensin-Converting Enzyme: Functions and Role in Disease, Nova Science Publishers (ISBN 978-1-53617-264-5) Chapter 13: 287-300
Nava-Anrade K., P. Knauth, Z. López, G.A. Hirata, S.J. Guevera and G.G. Carbajal (2020): Assembly of folate-carbon dots in GdDy-doped layered double hydroxides for targeted delivery of doxorubicin. Appl. Clay Sci., 192 ID105661: 7 pp.
Cervantes O., N. Casillasa, P. Knauth, Z. Lopez, A.Virgen, O. Lozano, I. Delgado, A.H. Sámano, S. Rosales, L. Martinez, V. Soto and M.E.Cano (2020): An easily prepared ferrofluid with high power absorption density and low cytotoxicity for biomedical applications. Mater. Chem. Phys., 245 ID122752: 10 pp.
Rodríguez R., M.A. Velasquillo, P. Knauth, Z. López, M. Moreno, J. Bravo, I. Jiménez, G. Luna and Z. García (2020): Sterilized chitosan-based composite hydrogels: physicochemical characterization and in vitro cytotoxicity. J. Biomed. Mater. Res. A, 108: 81-93
López Z., A. Femenia, G. Núñez, M. Salazar, M.E. Cano, T. Espino and P. Knauth (2019): In vitro immunomodulatory effect of food supplement from Aloe vera. Evid.-Based Compl. Alt. Med., ID5961742: 9 pp.
Knauth P., Z. López, G.J. Acevedo-Hernández and T. Espino (2018): Cinnamon Essential Oil: Chemical Composition and Biological Activities. In A. Cardador and V. Rodríguez (eds.), Essential Oils Production, Applications and Health Benefits, Nova Science Publishers (ISBN 978-1-53614-369-0) Chapter 13: 215-243
Knauth P., G.J. Acevedo, M.E. Cano, M. Gutiérrez and Z. López (2018): In vitro bioactivity of methanolic extracts from Amphipterygium adstringens, Chenopodium ambrosioides, Cirsium mexicanum, Eryngium carlinae and Pithecellobium dulce used in traditional medicine in Mexico. Evid.-Based Compl. Alt. Med., ID3610364: 11 pp.
Ocampo-Suarez I.B., Z. López, M. Calderón, J.A. Ragazzoz and P. Knauth (2017): Are biological control agents, isolated from tropical fruits, harmless to potential consumers? Food Chem. Toxicol., 109:1055-1062
López Z., G. Núñez, G. Avalos, G. Rivera, J. Salazar, J.A. Ramírez, B.A. Ayil and P. Knauth (2017): Antioxidant and cytotoxicological effects of Aloe vera food supplements. J. Food Qual., ID7636237: 10 pp.
López Z., J. Villarruel, J. Rico, T. Terrazas, J. Salazar and P. Knauth (2015): Cytotoxic effects and antimicrobial activity of Cuachalalate (Amphipterygium adstringens) extracts as used in a traditional way. J. Chem. Biol. Phys. Sci., Sec.B, 5: 1555-1565
López Z., P. Knauth, G. Avalos, V. Bocanegra, J.A. Ramírez, R. Reynoso and G. Rivera (2014): Cytotoxic effects of Sábila (Aloe vera): Commercial products as food supplement. J. Chem. Biol. Phys. Sci., Sec.C, 4: 47-54
López, Z., P. Knauth, V. Orozco and J. Salazar (2014): Manual de Prácticas y texto guía con conceptos teóricos básicos de Bases de Biología Celular. Editorial Amate, Guadalajara, 1st ed. ISBN 978-607-507-336-1
Solís, J., P. Knauth, Z. López, O. González, M.E. Macías, V. González, M. Lacroix and B. Aguilar (2014): Differences in the amount of β-glucan and mannan in strains of Saccharomyces cerevisiae and Meyerozyma guilliermondii isolated from agave must used in tequila production. Microbiol. Res. Int. 2: 1-8
Cano, M.E., T. Córdova, A. Hernández, P. Knauth, Z. López, M. Sabanero and M. Sosa (2012): Experimental setup for magnetic hyperthermia: pilot study. Rev. Mex. Fis. S58: 268-271
Aguilar, B., J. Solís, J.M. Viveros, Z. López and P. Knauth (2012): Characterization of cell wall extracts from Saccharomyces cerevisiae with immunological activity. Food Biotechnol. 26: 326-338
Cano, M.E., R. Castañeda-Priego, A. Barrera, J.C. Estrada, P. Knauth and M.A. Sosa (2012): Magnetisation of red blood cells: a Brownian dynamics simulation. Rev. Mex. Fis. 58: 391-395
Schlüter, T., P. Knauth, S. Wald, S. Boland and R. Bohnensack (2009): Numb3 is an endocytosis adaptor for the inflammation marker P-selectin. Biochem. Biophys. Res. Commun. 379: 909-913
Borges-Morales, R. (†) and P. Knauth (2008): Enzimas y Cinética Enzimática. Editorial Amate, Guadalajara, 1st ed. ISBN 978-970-764-506-6
Czubayko, M., P. Knauth, T. Schlüter, V. Florian and R. Bohnensack (2006): Sorting nexin 17, a non-self-assembling and a PtdIns(3)P high class affinity protein, interacts with the cerebral cavernous malformation related protein KRIT1. Biochem. Biophys. Res. Commun. 345: 1264-1272
Knauth, P., T. Schlüter, M. Czubayko, C. Kirsch, V. Florian, S. Schreckenberger, H. Hahn and R. Bohnensack (2005): Functions of sorting nexin 17 domains and recognition motif for P-selectin trafficking. J. Mol. Biol. 347: 813-825
Williams, R., T. Schlüter, M.S. Roberts, P. Knauth, R. Bohnensack and D.F. Cutler (2004): Sorting nexin 17 accelerates internalisation yet retards degradation of P-selectin. Mol. Biol. Cell 15: 3095-3105
Kruppa, K., S. Ziegler, P. Knauth, G.-P. Zauke and T. Höppner (2002): Bewertung polytropher Flachseen im Zusammenhang mit der EU-Wasserrahmenrichtlinie am Beispiel des Nonnensees auf Rügen. Bodden 12: 107-117
Knauth, P. and H. Reichenbach (2000): On the mechanism of action of the myxobacterial fungicide ambruticin. J. Antibiot. (Tokyo) 53: 1182-1190
| Projects: |
08/21 – 10/21: Responsible for Internship from DAAD/RISE: Topic: Cellular Effects of Functionalized SPIONs for Magnetic Hyperthermia 08/19 – 07/20: Co-responsible for a research project from SEP/Promep: Topic: Autofagía, apoptosis o necrosis: efecto de los polisacáridos a Aloe vera en células humanas. 08/19 – 10/19: Responsible for Internship from DAAD/RISE: Topic: Cytotoxicity of Epazote 11/15 – 10/16: Co-responsible for a research project from SEP/Promep: Topic: Determinación del efecto antioxidante, fototoxicológico y anti-inflamatorio de productos comerciales de Aloe vera 10/13 – 09/14: Co-responsible for a research project from SEP/Promep: Topic: Efectos citotóxicos y antimicrobianos de Guamúchil (Pithecellobium dulce) 08/13 – 07/15: Co-responsible for a applied research project from COTACyT: Topic: Efectos benéficos y toxicológicos de la Sábila (Aloe vera) y de sus productos comerciales elaborados en el estado de Tamaulipas 05/08 – 04/11: Responsible for a basic research project from CONACyT: Topic: Numb - the missing link in P-selectin internalisation? |
| Responsability: | since 07/2008: Responsible for the Cell Biology Laboratory |
| Scientific Stays: | 10/2010: Different Entities of the European Union in Brussels Training programme Foncicyt: Collaboration between Mexico and the EU within the 7th Frame Programe 06 – 08/2007: University of Barcelona Topic: Cloning of an Expression Vector for Rhamnolipids in a Heterologous Host |
| Formation: | - Course on Good Laboratory Practice by GlobalSTD (2018) - Course on HPLC in Pharmacy (2016) - Academic Teaching Excellence by the British Council (2015) - Course on Molecular Diagnostics (2012) |
| Projects: | 07/2005: Accepted project (as participant) by the company "Grupo Integra" and CONACyT (Fondo Economia): Topic: Implementing a process for making food supplements based on extracts of apricot (Prunus persica) and its commersalisation. - finally I could not participate due to my change to the University of Guadalajara - |
| Responsability: | Member of Safety Control in the Division Biotechnology (02/05 – 01/06) Member of the Safety and Hygiene Commission of the Centre (02/05 – 01/06) |
| Formation: | Course of patents at the Instituto Mexicano de la Propiedad Industrial (IMPI) |
| Objective: | Reveal the cell biological function of the sorting nexin 17 (SNX17). |
| Methods: | Molecular biological techniques: cloning, PCR, cDNA synthesis, sequencing, Western blot, yeast two-hybrid interactions, site-directed mutagenesis, siRNA Cell biological techniques: cell culture, siRNA, fluorescent microscopy (co-localisation), co-immunoprecipitation. |
| Results: | In a yeast-two-hybrid screen SNX17 was discovered by us as an interactor to the inflammation mediator P-selectin. Later on in cell culture studies we discovered a possible role for SNX17 in the endosomal sorting of P-selectin after it is internalised from the cell membrane. Now we want to reveal the influence of SNX17 on the speed of internalisation of P-selectin as well as the influence of a SNX17 knock out via siRNA. For those experiments I designed and constructed the necessary vectors and I did also some cell culture experiments. In two-hybrid studies I revealed that a FERM-like domain of SNX17 acts as the target binding part. By site-directed mutagenesis I could also discover the binding motif of SNX17 in P-selectin. |
| Projects: | 01 – 12/2002: Responsible of a 'Start-up' project for young researchers from the Magdeburger Forschungsverbund (NBL3) Topic: Which domains and sorting motifs of sorting nexin 17 (SNX17) are responsible for interactions to other proteins? |
| Responsability: | Responsable for Laboratory Safety (01/00 – 10/01) Principal Investigator (for genetic engineering) (10/01 – 12/04) in the spirit of the German law GenTG and GenTSV |
| Formation: | Course for online search in genetic databases @ NCBI (Entrez, PubMed, GenBank, UniGene, BLAST) and ExPASy (Swiss-Prot, TrEMBL) offered by BioTools |
| Objective: | Discover the mode of action of the fungicide ambruticin. |
| Methods: | Metabolic pathways with 14C-labelled precursors (scintillator), composition of lipids (TLC), permeability of the membrane, energy charge of the cell (AEC), analytics of glycerol and sugars (HPLC), cell biological and morphological alterations (e.g. protoplasts, liposomes, microscopy, antagonists). |
| Results: | The search for typical modes of action for antibiotics (like inhibition of DNA, RNA, protein or cell wall synthesis) were unfortunately unsuccessful as was the search for antagonists. Finally I could discover that the cells were getting leaky, the composition of the cell membrane changed and the cells accumulated glycerol. This was a clear hint that ambruticin is disturbing the stress signal cascade while the concrete protein, presumably a protein kinase, could finally not be isolated. A genetic approach was not performed, because the yeast Saccharomyces cerevisiae is resistant against ambruticin. |
| Formation: | Course for 'Responsable for Genetic Engineering' in the spirit of the German law §15(4) GenTSV |
| Objective: | Classification of the re-arisen shallow lake Nonnensee (Rügen) based on water chemical and biological investigations and proposals for a restoration. |
| Methods: | Organising the sampling, analysing nutrients like PO43-, NO3-, NO2-, Fe, Cl-, SO42-, hardness, pH, DIC, DOC, O2, BOD5, COD, Secchi-depth, chlorophyll, productivity, classification of plancton, 24-h-cycles (temperature, O2, PhAR). |
| Results: | Classification of the lake Nonnensee upon TGL 27.885/01- and OECD-guidelines as "highly eutroph" (polytroph). Unusual for a lake in the Baltic area was that nitrogen was the limiting nutrient and not phosphate. As the most urgent restoration act was supposed to construct a sewage based on helophyts to clean the waste water of a small village, which is entering the Nonnensee. |
| Formation: | Course about 'Risks and Hazards of Chemical Reagents' in the spirit of the German law ChemikalienVerbotsV §5(1) |
