Safety in the Laboratory

Material Safety Data Sheet (MSDS)

Material Safety Data Sheets (MSDS) or Sicherheitsdatenblätter (SDB) or Fichas de Datos de Seguridad (FDS) or Fiches de Données de Sécurité (FDS) contain safety instructions for using hazardous substances. Usually they follow this model:

01. Name of the substance or mixture
02. Hazards identification
03. Composition
04. First Aid measures
05. Fire fighting measures
06. Accidental release measures
07. Handling and storage
08. Exposure controls and personal protection
09. Physical and chemical properties
10. Stability and reactivity
11. Toxicological information
12. Ecological information
13. Disposal considerations
14. Transport information
15. Regulatory information (R- & S-phrases)
16. Other information

From this page you can download the MSDS of reagents present in the Cell Biology Laboratory (CBL). It is recommended also proof at the authors web pages, if these MSDS are still actual.
- Listing of International Chemical Safety Cards of the International Occupational Safety and Health Information Centre (CIS).
- Listing of International Chemical Safety Cards of the European Union (ICSCs).
- Meta-crawler to search for different MSDS (also in differents languages) is the euSDB - (Universität Mainz).

You can find here also MSDS for not hazardous substances, for special reagents used in molecular biology or cell culture.

Acetic acid, glacial
Acetic acid, 1 M
Acetone
Acrylamide-Bisacrylamide (37,5:1)
Adenine
Ammonium chloride (NH₄Cl)
Ammonium persulfate ((NH₄)₂S₂O₈)
3-Amino-1,2,4-triazole (3-AT)
Ampicillin * 3 H₂O
Amphotericin B

Boric acid

Calcium chloride (CaCl₂)
Camptothecein
Chloramphenicol
Cobalt(II)chloride (CoCl₂ * 6 H₂O)
Copper sulfate (CuSO₄ * 5 H₂O)
CTAB (Cetyltrimethyl ammonium bromide)
Cysteine
Cytoseal 60

10x DAB substrate kit
N,N-Dimethylformamide (DMF)
Dimethylsulfoxide (DMSO)
Dithiothreitol (DTT)

Ethidium bromide
EtOH abs

Formalin, 10%

Gentamicin SO₄

Histamine
Hydrochloric acid (HCl): 25% HCl; 1 M HCl
Hygromycin B

Immersion oil
Isopropanol
Isopropyl-β-D-thiogalactopyranoside (IPTG)

β-Mercaptoethanol
Methanol

Phenylmethylsulfonylfluoride (PMSF)

Saponine
Silver nitrate (AgNO₃)
Sodium carbonate (Na₂CO₃)
Sodium dodecyl sulfate (SDS)
Sodium hydroxide (NaOH): pellets; 10 M; 3 M; 1 M

Tetracycline HCl
N,N,N',N'-tetramethyl-ethane-1,2-diamine (TEMED)
Tris(hydroxymethyl)aminomethan (Tris)
Triton X100
Tyrosine
Trypan blue

XGal (5-Bromo-4-choro-3-indolyl-β-D-galactopyranoside)

Zeocin

The most substances used in the CBL are classificated as "not hazardous" in conformance with EC-directives & the GefStoffV and therefore MSDS are not obligatory. Although, they can be downloaded here for the following substances:
- Activated carbon
- Adenosine-triphosphate (ATP, 100 mM)
- Agarose
- Ammonium sulfate ([NH₄]₂SO₄)
- L-Arabinose
- Bovine Serum Albumin (BSA, 20 mg/ml)
- Bromphenol blue
- Coomassie Blue G250
- Copper sulfate soln (0.1 M = 16 g/l)
- DAB solution
- EDTA Na₂ * 2 H₂O
- Glucose
- Glycerol
- Glycine
- Glycogen (20 mg/ml)
- HEPES (free acid)
- Hydrochloric acid (HCl, 0.1 M)
- Isoleucine
- LB-broth
- Leucine
- Lithium acetate * 2 H₂O
- Lysine
- Kanamycin
- Methionine
- Magnesium sulfate (MgSO₄ * 7 H₂O)
- ONPG (2-Nitrophenyl-β-D-galactopyranoside)
- Phenylalanine
- Polyethylenglycol (PEG 3350)
- Potassium acetate
- Potassium chloride (KCl)
- Potassium dihydrogen phophate (KH₂PO₄)
- di-Potassium hydrogen phosphate (K₂HPO₄ * 3 H₂O)
- Proline
- Propidium iodide solution
- Sodium acetate
- Sodium chloride
- Sodium dihydrogen phosphate (NaH₂PO₄ * H₂O)
- di-Sodium hydrogen phosphate (Na₂HPO₄ * 2 H₂O)
- Sodium hydroxide (NaOH, 0.1 M)
- Sulfuric acid (H₂SO₄, 0.01 M)
- Tryptophan
- Turbofect Reagent
- Tween 20
- Uracil
- Urea
- Yeast extract

Also the enzymes and buffers used in the Molecular Biology area are usually not hazardous. These are:
- Buffer 10x B
- Buffer 10x BamHI
- Buffer 10x EcoRI
- Buffer 10x G
- Buffer 10x Ligase
- Buffer 10x O
- Buffer 10x R
- Buffer 10x Taq
- Buffer 10x Y
- DNA Ladder (1 kb)
- DNA Ladder (50 bp)
- DNA (Lambda)
- DNA (Salmon)
- dNTP Mix (2 mM)
- 6x Loading dye (w/o SDS)
- Phosphatase FastAP
- PNK
- Polymerase Pfu
- Polymerase Taq
- Proteinase K
- RE Acc65I
- RE BamHI
- RE BglII
- RE Bsp120I
- RE Bsp1407I
- RE Eco32I
- RE Eco72I
- RE EcoRI
- RE HindIII
- RE NcoI
- RE NdeI
- RE NheI
- RE NotI
- RE PstI
- RE PvuII
- RE SalI
- RE SmaI
- RE XbaI
- RE XhoI
- RNase A
- T4 DNA Ligase

The media and additives used in the cell culture are not hazardous - with exception of the antibiotics:
- Animal Cell Culture
- DMEM high (Dulbecco's Modified Eagle's Medium)
- DPBS (Dulbecco’s Phosphate Buffered Saline)
- FBS (Fetal Bovine Serum)
- G418 SO₄
- 100x ITS (Insulin, Transferrin, Selenium)
- 10x PBS (Phosphate Buffered Saline)
- Trypsin EDTA

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Last modified: 17.02.2011